FastQCFastQC Report
Wed 6 Jul 2016
502749_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502749_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128498
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACATA100.00269215581738.6063235
ATAATTT150.0060562041159.0708235
AGGGGGG954.3541545E-4366.02237235
TATGCAT100.0021334682216.564442
TATTAGA100.0021334682216.564442
TTATTAG100.0021334682216.564441
CCATATG150.002608748202.45778220-224
TGTATGT450.00196521172.188144
CTATCAA650.00842977649.9764064
GCACTGT1602.0754973E-433.8381926
GGGGGGG5301.1746306E-732.80389235
TTTTTTT300.005324041529.791378120-124
TTCCCCC1250.001458427924.25786230-234
TTGATGC2350.001964126723.0387674
GTGCTGA2800.005424276519.3361111
GCACCGT4600.005563142714.1237666
AACACCG7155.886792E-412.1154935
GTGCTGG8501.9401913E-411.4651751