FastQCFastQC Report
Wed 6 Jul 2016
502749_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502749_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128498
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATAA100.008801521962.79626235
TCCATAA150.00660803148.41082
ATGTATA150.00660803148.41081
TTCTCAT150.00660803148.41085
GTCCATA356.4931717E-495.4069441
GGATAGT450.00176165974.20546
ATAGAAG550.003903109360.713513
CTTTACG650.00755966351.3729672
GGTGATT1650.00860760826.983781
CCCAGGG2200.001139330625.2972972
GTGCGAT1850.00404245459.62664630-34