FastQCFastQC Report
Wed 6 Jul 2016
501725_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501725_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153209
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA57.256129E-43351.1123235
TAATTAA57.256129E-43351.1123235
ACTAAAA100.00290188031675.5562235
TACTTAA150.00652794451117.0375235
TTATGTA150.00652794451117.0375235
CGAAGGG1550.002114193216.20078235
TTAGCTT450.00204712971.455394
ACGTCTG956.915371E-445.1297237
AACAAAA500.00460580830.68526225-229
TCAAGAC1804.4008606E-429.773083
GCTACAC1500.006503741728.5821593
GTACTGA1600.00891142826.7957741
TCTCTTA1100.00989096217.434807225-229
CTCTTAT1100.00989096217.434807225-229
GAGCGGC4600.00595936213.9804046
ACAAGCT1450.00820604810.598454220-224
GGGGGGG3603.9260565E-59.602602230-234