FastQCFastQC Report
Wed 6 Jul 2016
501725_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501725_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153209
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTAA100.0020339733220.061365
GGCAGGG2900.00103192984.65032235
GTATAGG400.001155942782.5231
ATAAGAC450.001844894873.353793
GTATTGA1150.001556049571.15534235
CCTTAGC500.002801735466.01849
TAAGACG550.00408717960.016734
GCCTAGG650.007915501550.783392
CCTACAC904.6551987E-448.9025273
TGGACTG1905.180154E-428.9554425
TTTTTTT406.9702487E-427.54002645-49
TGGACCA2958.478415E-626.1089765
GTGTGGG2801.7873422E-423.5780051
TGAAATT650.00984605617.450632100-104
AATCCTG1550.00998927510.317413110-114