FastQCFastQC Report
Wed 6 Jul 2016
500523_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500523_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134161
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAAGA100.00237401321851.2742235
CTAATGA150.00534058041234.1827235
GAATAAA200.009492678925.6371235
TACATTT200.009492678925.6371235
TTACATA100.0022140949213.900483
AATTGAG150.0028526885196.50574215-219
TAAAACT150.0074464595142.600314
TATCTAT150.0074464595142.600311
CCTTTAA150.0074464595142.600311
TATTACG400.001293549580.2126852
TGTCTAG450.00206430371.300162
CTACATG600.006451648753.475124
AGATATA450.00770480351.135612230-234
AGTTCTC1100.00876498549.46802225-229
TAACCCC650.00885320349.361654
GTACATG1100.001437357938.8909951
GTACCTC1150.001788158837.2000851
GTCCAGT1602.2325187E-433.421951
CTGGAGC2150.001257953824.8721494
TGGAGCG2550.003391861220.9706365