FastQCFastQC Report
Wed 6 Jul 2016
500523_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500523_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134161
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAA50.00331084081570.5703235
TATATGG400.001142658382.757872
GTCCATA650.00782496550.927921
GGCGAGG4700.00822247750.12458235
CCAGGCG4900.00969441648.078682235
GTATGGC955.990015E-446.460561
AAATTTC350.004573667430.730762220-224
TTGCCCT1955.9270975E-428.293294
ACACCTG1600.007730160427.5859585
CCAGATG3103.4882472E-421.356873
TGAGCAG3150.00960001317.5148945
GTGTTCG4350.003386612715.219841
GTCCAGG4800.006511216513.7929781
CCGCGCA6903.7223878E-412.79348859
CTGATCG6350.00290923912.1638864