FastQCFastQC Report
Wed 6 Jul 2016
500519_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500519_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132852
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAGG200.0079857571008.8785235
CTCATAC300.004714329166.07054230-234
AATGATA150.0075796144141.756876
AACTAAT150.0075796144141.756875
TAACTAA150.0075796144141.756874
GTGGGGG2850.0074530444141.597235
CTCCAGA400.001324348379.7382351
CTAGACC450.002113405870.878444
TACTACG701.593314E-460.7529452
CGTAATG550.004680861357.9914443
TCCATAT650.009062991549.0696832
TACCGTT650.009062991549.0696837
AAATGAC650.009062991549.0696832
GGGGGGG5053.90449E-639.95559235
TACCCAG1300.003358824932.7131235
AGAACAT1300.003358824932.7131235
TAGACGA1400.00482751130.3764724
GTCCAAG1500.006763207328.3513741
CCCCCCC2503.6881564E-412.818423225-229
TGGCGGG4100.003662783712.1515045230-234