FastQCFastQC Report
Wed 6 Jul 2016
500519_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500519_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132852
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGG150.0069188136146.148794
GGTAATT356.902437E-493.952793
GTAACTG802.6511174E-454.8057983
GGTCGTA1450.004936850630.237681
TTGAGGC1550.006838856728.2868633
AAAAAAA659.380257E-426.17164230-234
ACCGTGC5203.5455174E-516.8633218
CCGTGCG5206.7823904E-414.7554079
ACCGCAC4500.00443496114.6148798