FastQCFastQC Report
Wed 6 Jul 2016
500024_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500024_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132749
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAA200.0069189671083.3536235
AACTAAA200.0069189671083.3536235
ACTCTTA400.007138709144.5255230-234
GTCTCTT1550.0010649803139.78757235
TGTAATA150.008990415133.888472
TCTAGAA252.5652832E-4120.4996262
GGGGGGG3501.2223609E-961.90592235
CATTAAC500.00402373360.2498139
TAGAAGG600.00827762450.2081764
TATCGAA1150.002436436534.9274252
GTTCGAG1857.559969E-427.1395551
GTTCTAC1908.83422E-426.4253541
GTGTTGG5050.001054843413.9190981