FastQCFastQC Report
Wed 6 Jul 2016
500024_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500024_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132749
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAA150.008274947137.65511
TAAACTC150.008274947137.65515
GTCTAAT407.404378E-8129.051671
GTGTTAG600.00741780251.6206631
GTCTTCT600.00741780251.6206631
CATGAAC1350.00466487630.5900253
TTTACGA650.00694194218.540741150-154