FastQCFastQC Report
Wed 6 Jul 2016
500005_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500005_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141356
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAA200.0065483351113.8779235
TTGGGCG3050.006779535146.08235235
TAGACAG150.008277822137.642275
GGTATAT150.008277822137.642272
CCGCGGG7006.201266E-495.47524235
AAGACTG450.002375590168.821145
ATACGTT450.002375590168.821144
GGGGGGG5305.7862053E-942.033127235
GGTTACG1050.001361071539.326361
AGTCGTT1450.006619362628.4777076
AGGACCT1500.00781067627.528455
TCTTATA750.002645436721.902208215-219
TGTCTCT1101.241296E-418.45328205-209