FastQCFastQC Report
Wed 6 Jul 2016
500005_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500005_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141356
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAA50.00269953441739.1589235
AGCTTAG150.007556463141.90521
AGAGTGA150.007556463141.90521
TTCTATG357.7609904E-491.224772
CTCATGA650.00902680349.1210331
AGATATT300.006376506328.71213765-69
TTCTGCA1600.00922122126.6072252
TCTGGTC2751.9810705E-423.2208528
TACATGT550.0048904519.70053775-79
TGCACCG3951.0430754E-418.8608175
CAACGCG6400.0074832885.1604433180-184