FastQCFastQC Report
Tue 5 Jul 2016
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428496
Sequences flagged as poor quality0
Sequence length32-241
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTAA900.00787210450.867065235
AAATCTC1457.545802E-540.213657
CAAACCT1200.001444133938.8731961
AGACTGG2403.003327E-431.791916235
GTGTGGG12800.029.80492235
TTGTTTT2150.006731510628.390919235
GATAAGG3652.8258582E-522.36541
CAGGAGT3454.967784E-420.2816684
AAGGAAT4200.001861522716.659944
AGGAATA5053.4121907E-416.165095
ATAAGGA5153.9615884E-415.8512062
AGGAGTA4700.003941096414.8876081
TGGAGGG6350.00487089714.419011235
TGAGTAA9752.8502485E-514.086264235
CCAGCGG5000.005943089313.994358
AGAACGG6800.00766707813.46481235
TGGTGGG11357.953189E-613.445039235
TTAAGAC1400.003815384311.744209140-144
GTTGGGT3252.6739144E-911.676355220-224
TGGGGGG21754.8839865E-911.225835235