FastQCFastQC Report
Tue 5 Jul 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428496
Sequences flagged as poor quality0
Sequence length32-241
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG251.4225181E-4139.788169
TACCCTA550.003264489463.5400776
CCCTATA550.003264489463.5400778
TATACCC751.4291304E-462.1353264
GTCTATG700.00847017349.9359971
ATTCTAC700.00847408249.9301723
ACCCTAT700.00847799249.9243437
CACACTA1000.00902689949.134598235
CTAGAGC1005.919265E-446.5960548
TTGCTAG1057.527747E-444.3771935
TGCTAGA1109.465266E-442.360056
GTTCCTT1300.002148789435.851491
GTGGGTG6900.033.231133235
AAATCTC4351.7644197E-1029.4572755
GAAATCT4502.6193447E-1028.4786914
TCTACCG2106.6827633E-427.7357465
GCTAGAG1700.00799535427.4094437
ACCTAGA600.005132217419.539082125-129
CCTGATC3850.001046950718.1543068
AGTGTGT1254.774147E-617.019302195-199