FastQCFastQC Report
Tue 5 Jul 2016
MU572163T_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU572163T_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100666
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGT450.001590725676.126686
TACTCTC500.002416067568.5140155
GAGGTCT550.003525038562.285466
CTATGCG600.00497510657.095014
TCTTAGG852.918665E-453.7364772
GGTCCAG650.006828656452.7030836
ACTGTCG650.006828656452.7030838
GTCTAGC955.05303E-448.080011
GTGTGTA955.05303E-448.080019
GTAGTAA1601.5165606E-435.684381
TATACAG1300.002363801735.1353875
TCTGAAG1450.00403650431.5006923
GTATAAT1550.005593352529.4683931
TTATCCT1600.00653187928.5475034
GTGTTCA1650.007590709327.6824281
ATCGACT1650.007590709327.6824288
ATATAGC1700.0087807626.8682388
GATATGG1700.0087807626.8682381
ATAAAGG2901.7572619E-423.625523
TTCTGAA3950.001411083317.3453182