FastQCFastQC Report
Tue 5 Jul 2016
MU572163T_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU572163T_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100666
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT802.1641182E-457.090661
TATACCT650.00683071252.699074
ACAGACC700.00915565248.9348538
GACTCAT700.00915565248.9348531
ATACAGT1304.463997E-543.9158946
TATAACT1200.001596306838.060444
ATACTGT1803.030298E-431.7170336
CTATAAT1500.00476658830.448351
TACAACA1550.00559543529.4661484
TAATATT1954.843914E-429.2772624
AGTACAG1650.007593529827.6803235
GTACAAA1700.00878401726.8661941
GAAATGC2159.470092E-49.666938100-104