FastQCFastQC Report
Tue 5 Jul 2016
BS20102N_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS20102N_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105727
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACT251.4905319E-4138.085141
CCTAGAT550.00341980762.7659723
CGGTGAA751.5140697E-461.371187
TTAGGCT1150.001246816140.024684
GTATTAG1350.002739824834.0950971
TAGATAG1702.0707841E-433.8444025
TATGAGC1450.003888349731.7437113
ACATAAG1600.006292646828.7677381
GACTATG1750.00974391326.3019337
GGTTTAT2400.001556679323.9731161
AGATAGC2600.00247939122.129036
GGGGGGG550.002617231321.943386200-204
GTATTAT2650.002769324221.71151
TGTAAAG2700.003086561321.3094352
TTAGACA2800.003810907520.5483864
TATAATA3556.544989E-419.4486122
CGCAGAA650.00733668918.363403180-184
CTTAAAG3200.00824397317.9798373
ATATTAT4150.001866334916.6367651
ATTTTAT5355.8062444E-415.0560131