FastQCFastQC Report
Tue 5 Jul 2016
BS20102N_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS20102N_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105727
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG100.0017817464229.979663
GCGGTTT950.00784619750.8713235
TCTAGGG700.00890514449.2813533
CTATACT1006.288091E-445.9959344
GGTATAT1100.001005423741.8144841
GTTATAG1100.001005423741.8144841
ATCCACT1200.001542266538.3299454
GTATTGG1951.11980535E-535.3814851
ATAACAC2351.0183558E-634.252293
GAACTAG1400.003285718232.8542371
TTATACC1752.4652394E-432.8542374
TAAGATG1450.003901797331.721335
GTTCATG1500.00460591330.6639561
CATAAGG1550.00540695929.6747933
TAATATA1600.00631436428.7474582
AATATAT1700.00848877327.056433
TTCATGG1750.00977746726.2833882
TATTGAC2659.091338E-526.0354333
TTATTCT2750.003447130320.9072424
CAGTCTC600.0057176819.17404235-39