FastQCFastQC Report
Tue 5 Jul 2016
607239_S16_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename607239_S16_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259941
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTTA100.0021218748217.001542
CTGTTAT357.1953994E-493.0006561
GTATACA750.001427869978.24408235
TACGTAT550.00432357559.1822367
TGTTATG802.792882E-454.2503852
TGTTAGA600.00610098854.2503852
GGCCTAG2000.001396459439.12204235
ATAGACA1150.00166898937.73943
CCTCTTA300.001917771136.696815225-229
TGTAGAA1250.002512990534.7202452
CCCTGTA1250.002512990534.7202452
TTTCATA800.002547307234.62904230-234
CTTATAA1750.00848757933.533176235
GTATCAG1400.004378213531.000221
GTTATGG1500.00613467328.9335373
ATCTACC1650.00976727926.3032172
TTGGGTT2150.001159627125.2327384
CTACTGG2850.005954809519.0352231
TTGGCCT4201.4461283E-418.083464
GTCTTGG4251.583271E-417.8707161