FastQCFastQC Report
Tue 5 Jul 2016
606111_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename606111_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183613
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC500.002556080567.5698553
TACACAA950.004655652758.06475235
CTATCAG600.005262871356.308211
GTTATTG600.005262871356.308212
CCCTGTA650.00722325751.976817
TATTGAT700.00968130748.2641834
GGTTATT700.00968130748.2641831
GTACACC2851.7234078E-423.708724
CTCCAGG2800.004329648320.1100771
GTGCTGT2850.004796572519.7572651
GGGAGGG4850.00607925218.955847235
TACACCG3659.155648E-418.5122875
GCGAGGG5000.007247787418.387173235
TACACAC700.008336375517.962587215-219
TCGGGGG5200.00908630317.679974235
TGCTGGA5803.6745078E-617.4749622
TTATAAA900.001655074216.957926225-229
GTCTTGG4000.001691714916.8924641
TGGTGGT6301.4537017E-414.3004985
TCTTATA1901.2373192E-412.049052225-229