FastQCFastQC Report
Tue 5 Jul 2016
606111_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename606111_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183613
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATATA100.0019209016224.318911
TTAAAGT100.0019209016224.318912
ATATATA150.0064614336149.545932
TATAGGA356.3038094E-496.1366652
TACGCAT751.7220026E-459.818377
ACGACAG2350.00240353735.016003235
TCCATGA1500.005212830429.9091852
GATACCC2057.227751E-427.3559654
ACAACGG3304.774013E-420.3926263
CAGGGTG4953.9278402E-415.8609325
GTCCAGC7353.6012832E-513.7338091
GTGTTGG5000.007671606313.4591331
GGGGTGC5150.00932624713.067126
GGACCGC6650.003646205211.8062576
CGTCTGA1450.006978237510.82918810-14
ACCGGGC9150.00344956539.8062928
ACCGCGC11452.0024013E-49.7955858