FastQCFastQC Report
Tue 5 Jul 2016
506776_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506776_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219386
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAGA100.0019329559223.860472
TCATATA150.006501934149.240312
TGTAGTG600.00539310955.9651155
GTCATAT650.007401842651.6601071
CTATACC904.2823693E-449.7467734
TGTACAG1059.1534253E-442.6400875
ACTGAAC1150.001431871938.9322553
TATACCG1150.001431871938.9322555
TAGACGA1200.00176486137.3100786
TAACGTC1600.00721977927.982565
CGTAGAA1650.0083894727.13462
TGAACAT1700.00970398726.3365253
GTGTACA1700.00970398726.3365251
CAGTGCA2558.4481006E-526.3365259
GTAGACG2500.002326443822.3860474
GGTCTAC2750.004043716520.350951
ATGCAGG2800.004488274519.9875411
ACAGTGC2850.004972186419.6368838
GCTGGTA3200.009700865517.48912
GTCTTGG4250.002644366815.8019151