FastQCFastQC Report
Tue 5 Jul 2016
506776_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506776_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219386
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGAG100.0019441422223.429343
TCAATAT400.001088910783.7863
TAAGGGT450.001738004474.476444
ATCTTTT900.00244607368.309006235
CTAAGCA600.005434407455.857334
GTTCTAT700.00999627547.8777121
GTCTACG2001.6312044E-533.51441
CCCTGTG1500.00531731529.7905751
GTACGAG1600.007288038327.9286671
GTAAAAA500.00876871226.89799230-234
CCGTGTC3050.007438669418.3138799
GGCATGA3200.00980911417.4554166
CACACTG3200.00980911417.4554165
TCTGGGC4150.002285063516.1515182
TCCTGGG4600.00453594814.5714772
TTTTTTT1650.004358730712.41982235
TGGGCCG8651.7185044E-411.623495
ACTTCTT1650.00890063410.479986185-189
GGGGGAT2250.00390479579.667885230-234
CCCCCCC5452.2732762E-46.3456793185-189