FastQCFastQC Report
Tue 5 Jul 2016
506683_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506683_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136964
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA150.0063960757150.04142
TAGTTCA303.3682704E-4112.531054
CTAGGGA303.3682704E-4112.531054
TTCTATA356.2180485E-496.455192
TCCTGTA500.002562378567.518632
CCTGTAC650.007241025651.937413
GTATTGA700.00970510948.2275961
CTCTGAC700.00970510948.2275963
ATACACC1459.278397E-538.803814
CCTACAC1250.002097799236.0099373
GTGTAGG1400.003656386432.151731
TACACCG2653.0882566E-629.7251855
TCCTGGG2507.0998474E-527.0074522
GTAGGTC1700.00944185426.4778944
AAATGTG350.00973440226.327385100-104
TTGGGCC2850.00481150219.742294
GTGTTGG3900.001432685517.312471
AAAAAAA1954.7693902E-815.4497215-219
ACGGAGT1150.00958420213.020122210-214
CCTGGGC6400.002663729712.3080843