FastQCFastQC Report
Tue 5 Jul 2016
505907_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505907_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248169
Sequences flagged as poor quality0
Sequence length32-241
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGTG1552.7037622E-643.551355
GGCCTAG1250.002103229836.002451
GTATGAT2102.1710637E-532.1450461
CTATTAG1752.8129248E-432.1450461
TGTTTCG1500.005137862630.002047
CTGTCTT2400.00536896429.726465235
CAGTACA2159.383885E-426.1645744
TATACAG2400.001781507923.4390965
ATACAGA3052.7497704E-422.1326546
TATAGTG2600.002836464421.636095
TAATACT4755.4921657E-721.317244
ATACTCT2700.003530443220.8347516
GTATATA5101.0871572E-619.8542921
TTAATAC4559.0255E-619.7815673
ATACTGT2950.00589324519.0690946
CGGGGAG4800.006838110718.57904235
CTGCTGT3750.001106385118.0012269
TATACTG3150.00860416217.8583585
AAGCCAG3200.00942109117.579325
ATATAGA7551.5191472E-716.3918441