FastQCFastQC Report
Tue 5 Jul 2016
505907_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505907_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248169
Sequences flagged as poor quality0
Sequence length32-241
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC1752.841427E-432.0898553
TATATGC3008.262958E-626.2067133
TATACAG2200.001083913525.5260222
GTACAGG2300.001404517724.4161931
GATAAAC2600.002864890321.5989422
TATACCT4907.5283606E-720.6291924
ATATAGG3405.7918864E-419.8202041
TATGCTG3608.508585E-418.7190825
TTATGCT3100.0079243218.115244
GTATAAT5052.2497608E-517.7923971
GGGCAGG5300.00926398317.625174235
GTATTAA4652.417595E-416.9075581
GTATATA4853.333216E-416.2103391
ATATTAT11552.7284841E-1115.5587181
ATATGCT4400.003258478115.3156134
GATAATA5155.2619196E-415.26604752
GCTTTAT5155.2619196E-415.26604751
TATTATG5205.662475E-415.1192592
TCGGGCC5357.025703E-414.69535354
GTTTATA5000.007611745513.4777381