FastQCFastQC Report
Tue 5 Jul 2016
505137_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505137_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136505
Sequences flagged as poor quality0
Sequence length32-241
%GC61

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATC450.001280938980.40911190-194
TAGACCC500.00242760668.444955
GGTTTAG500.00242760668.444951
TGCTTTG1159.8920755E-566.86516235
TATACAA550.00354185462.222685
TGAGGGA650.006861157752.6499565
CAATGGG1150.00830475550.148872235
AACTATG700.00919641448.8892487
TTGAGGG1100.001046802741.4817854
GGACAAC2005.6587235E-428.5187286
ATAAAGA1650.007637543627.6545223
GTACAGC2600.00261163421.9374831
GTCCACG3556.953624E-419.2802661
CCCCGTT1050.00919954213.10143965-69
CCGTGCG6350.002267298512.5751879