FastQCFastQC Report
Tue 5 Jul 2016
504782_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504782_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207671
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACAAA200.003084765191.45532235
ACCCTAA150.006496703149.278581
TAACTTG354.190225E-4106.53632200-204
CAGAGGG1402.6752515E-454.70152235
ACTCTGT650.007393789551.6733556
TACTCAT650.007393789551.6733555
TTGAATA700.00990966147.98243
AGGACTC955.583097E-447.1406065
TAGGACA955.583097E-447.1406064
TACTGCA1253.991767E-544.7835732
CTTTGTG1350.003140819733.1730162
TATGACG1752.8931609E-431.9882684
CAAAGTG1400.003753225631.9882667
TAGCGTC1450.00445651230.8852237
CATACCC1550.006174408428.8926284
GCGAGGG4704.263822E-628.514622235
GTACTGC2754.3017953E-628.4986381
GTGTACG1700.00969070126.3432791
TCCTGGA2950.00605877718.9760912
GGCAGGG7101.0351734E-418.875875235