FastQCFastQC Report
Tue 5 Jul 2016
504782_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504782_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207671
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATA205.918233E-6310.09592235
TATAAAA409.3963325E-5155.04796235
AATTGGG900.002362158468.9102235
CCATAAT550.003861997760.8893851
GCATATG600.00545022255.8152732
ATCAGTC1250.00218476235.7217753
GTGTAGG1300.002648233434.347861
TCCGATA1350.003186352633.0757142
CAGGACT1702.48303E-432.8325164
CATCAGT1500.005336282329.7681452
CCCTTGA1600.00731427.9076371
TAGACCG1650.008498874527.0619495
GAGGTCC2550.002650465821.888346
GTGTCAC2700.00369224120.6723211
GTCCAGA2850.005048984719.5843051
ATTCCGC3050.00746974618.3000896
GTTCTTG4150.002296031216.1393551
GTCTTGG4150.002296031216.1393551
CGATTCC4750.005639235514.1006994
GATGTGG4900.006929007413.6690451