FastQCFastQC Report
Tue 5 Jul 2016
504302_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504302_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305211
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAT550.00487220257.4254763
CCCTAGT600.006874379252.640022
TATAGGT650.00943270548.5907864
TAGGAGT1100.001556052538.283654
TTCTACA1150.001935694636.6191442
AGAGGGG2400.001963789336.499233235
GTATAGC1200.002385289435.0933461
TGTAGGC1804.898836E-429.2444553
CTTATAA3000.001423691229.199387235
GTGTACT1450.006024399329.0427681
AGGAGGG3050.006351057428.720709235
TAGTCCA1500.007109162428.0746779
TCTATCG1500.007109162428.0746773
CGTTTAC1550.0083427527.169042
CTACAGC2600.00417009120.246163
CCTGAAG4001.2544352E-418.4240063
CTCCTGG4101.5156297E-417.974641
TTCGGGA4100.003134313315.4068352
TACACAA1500.001718648614.599693235
AAACACA3100.004598034514.128736235