FastQCFastQC Report
Tue 5 Jul 2016
504302_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504302_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305211
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAC355.5872406E-6119.4275363
ATTAGAG450.002265898369.666075
ACTTATA500.003440421562.6994551
TATTAGA550.005017922356.9995084
CTGTCTT1350.0058079654.91378235
TTAGAGG600.0070797852.2495546
TTATAGC650.00971427248.2303542
CCCCCTA650.00971427248.2303541
GTTATAG650.00971427248.2303541
CATAGAC1050.001284159739.8091773
TCATAGA1050.001284159739.8091772
AGAGGGT1200.00247400934.8330358
ATGGACT2053.0522668E-530.58514
TACCGTG2757.3681367E-626.599777
AAGAGGT2200.001650955123.7497944
GTACTGG4301.0495389E-519.4416921
GTGTTCA4351.1596459E-519.2182251
TCCTGGA4351.1596459E-519.2182252
GTATTGC3307.7037455E-418.9998361
GTGTCAG2850.007400093618.3331741