FastQCFastQC Report
Tue 5 Jul 2016
503710_S18_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename503710_S18_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181057
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT150.0061720414151.854743
TTATACT150.0061720414151.854744
TATACTC150.0061720414151.854745
TAATGCT500.002444474368.3346254
GTATACG550.003566439762.1223951
GCATACA1300.002399441835.04342
GTACCTG1400.003450398632.5403021
CAGGACT1904.2360844E-429.9713294
AGGACTG1600.006629827428.4727635
GTCGTAG3855.0973096E-520.7074621
AGGGGGG5254.8405636E-420.341549235
GGGAGGG4700.006119190718.934954235
GGACTGG4050.001704861516.8727476
TATACAC1052.959503E-416.472918225-229
GGGCGGG14055.6779754E-815.20187235
TCCAGCA4950.006485379313.80497552
TTATACA1100.007631195713.477841225-229
AAAATGG1100.00831162113.304854205-209
GGGGGGG28400.013.161126235
GTCCAGC7100.005301499311.2286961