FastQCFastQC Report
Tue 5 Jul 2016
503710_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename503710_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181057
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAA150.0012083538261.83235
TTAGCAC500.002472773768.1365363
CAATAGG550.003607691761.9423031
TTTACCC650.00698845952.412724
ATAGAGG650.00698845952.412723
ATAGGAG700.00936688448.6689534
TAGCACC1200.001643078637.853634
GGTAGCA1600.006724485728.3902242
GTATTGG1700.00903924726.7202111
ACCAAGC2900.00505375619.5794648
CCGGGGG7650.003122809315.401764235
CTGGTCA5406.9211645E-414.7208589
ACCGTCA6251.2651643E-414.5357948
GTGCTGA5558.517513E-414.3229951
TGGGCTG4800.005391584214.1951115
CTGGGCT4850.005775104714.048774
CGGTACC5000.0070660813.6273084
ACATCGC5150.00859134413.2303958
CCTGGTC7250.006329623510.96458
CACCGTC11902.2515187E-510.4972257