FastQCFastQC Report
Tue 5 Jul 2016
502836_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502836_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202478
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAG250.0075287786142.07506235
TTTAGGG701.1371642E-465.05593
ATAAGTG600.00504190256.9239165
TAGAGCC955.140758E-447.9359254
TATTGGA955.140758E-447.9359252
CTATGGC1450.00410595431.4062964
TATTGGT1650.00772083627.5994742
TACGCTG1650.00772083627.5994745
TACACCG3355.68416E-727.1875425
GTGTAGG2158.7515E-426.476241
GTATTGG2300.001296995724.7495271
GGGGAGG6503.781499E-621.8577235
TGGGGGG9452.3960638E-718.792997235
ACAACGA4950.0083633217.93877235
TGCGGGG7952.1530876E-517.871077235
CGTGGGG5100.00993224917.41116235
CTACACC4100.001856416316.6606584
TCAGCAC5154.741419E-415.4744623
ACCGTGC6300.002175093212.6497588
ATTGCGT1500.00683691410.859184110-114