FastQCFastQC Report
Tue 5 Jul 2016
502749_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502749_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189182
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTA700.00928049548.784072
TTACGCC1058.422851E-443.3636174
GTACCAC1500.004849740330.3545326
ATCACAC1500.004849740330.3545329
CCTACAC1550.005692961629.3753553
CTCTTAA350.00593852429.134129230-234
GTCTTGA1700.0089367126.7834119
CTCCTGG3109.589245E-625.7034361
GGACTGC3102.8328333E-422.0315156
CCAAATG703.6653673E-421.219976215-219
ACCGTGC6501.7312494E-414.0097858
TCTCTTA1100.007535693213.503622215-219
CTCTTAT1300.001472976313.3305215-219
CCGTGCT5150.00846011913.2616899
GTGTTCG6400.002450041512.4501021
CGCGGGG13400.001363769810.95843235
AGCACCG9603.7898458E-410.6715155
CATCACC9300.00349290029.7917854
TGGGGGG7500.00196434289.789532235
GCGGGGG21056.0217157E-59.591881235