FastQCFastQC Report
Tue 5 Jul 2016
502749_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502749_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189182
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACA600.005087489756.7931564
TATGACA700.00935924848.6798484
ATGACAG1200.001641459237.8621035
GTATTTC1250.002005797336.347621
TATGGCC1250.002005797336.347624
TAGCATC1350.002925717733.65524
GTGCAAG1803.1349412E-431.551751
TTGAGCA4006.9849004E-519.8776044
CCTTGAG3200.00890975717.747862
ACCGCGT6200.001961452412.8242618
AGCACCG10405.0350845E-612.0139365
AGCAGCG9450.00405787869.6157725
CAGCACC15604.4916014E-58.7374094
AAAAAAA5203.6573148E-48.7374086
GTGCTGG12650.00479149548.081241
GGGGGGG29050.00108374667.3391066235