FastQCFastQC Report
Tue 5 Jul 2016
501725_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501725_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223795
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA600.00532557656.1439744
TGTAGAA1152.397618E-548.8208472
ACTCAAT955.503835E-447.2791373
GTGTAGA1300.00257349234.550141
TCCAATG1400.00370029332.082272
ACATGGG2500.005599577429.4691235
TACTGCT1550.006087518828.9775355
AGTACCA1700.00955463326.4206925
GTACTCC2600.002867946421.5938384
ACTGATC3659.3457726E-418.4582928
CAGTACC3900.001457504317.2750684
GGGGGGG27800.017.225643235
ACTCCGC3950.001587288917.0563988
TGGGGGG6800.002002337516.25134235
ACTGCTG5000.00762056413.4745536
TCCAGGT5000.00762056413.4745532
CTCCGGG7254.898773E-412.3903952
ACCGTGC6900.00476792511.3915318
GTCCAGG7100.0058991411.0706421
CCGGGGG12200.00830461110.567814235