FastQCFastQC Report
Tue 5 Jul 2016
501725_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501725_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223795
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA150.0011872522263.3969235
CCTTTAG250.0054757455158.03815235
TCTTAAG150.006482079149.39372
CTGTCTT1251.3088138E-463.215263235
GATTTAG650.00737203151.7132031
TTCAATA700.00988052348.01942
GTTATGG1007.1625813E-444.8181081
CTTGATA1100.001145021640.7437364
ACAGAAA1350.003129492733.19868
TAGAACT1400.003739732.0129364
AGGGGAG1752.880814E-432.0129365
TAGGTGT1500.005241269729.8787384
GTGGTAC2751.4013931E-424.4462411
CCTTGTG2550.002594635121.969662
CCATACC2750.00401997920.3718684
ATCATCC2750.00401997920.3718683
GTACTGG4951.9323139E-518.1083281
CCCCTGG3100.00803229918.0718171
ACTGCTC3800.001242527717.6913598
CAAGACC5703.2740081E-617.6913594