FastQCFastQC Report
Tue 5 Jul 2016
500523_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500523_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207110
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGT150.006270573151.057724
TAATGGG600.005140451756.6466452
GTATCAC1200.00166278337.764431
TCATGAT1500.00496410530.2115442
GTATTGG1550.005827107529.2369791
GTGCAAG2354.4591696E-528.9259451
GGTGATT2750.00836217427.143848235
GGAGGAT1700.00914680926.6572445
ATGGTGT2801.4684675E-424.2771326
CTATCCG2600.00272268721.787179
ATTGGGC2850.00463510719.8760173
GTTCTGG3658.7995175E-418.6235541
TCGGGGG8201.8342445E-518.20624235
TGGGGGG7201.4028403E-418.143024235
TCTTATA1602.53359E-514.269247225-229
CTCTTAT1300.001225710513.659451225-229
TTTACCG1500.003972926611.679675210-214
TCCAGCA7150.00581400711.091652
CAGGATC7200.006123487411.0146264
TGTCTCT1700.009120163510.445462225-229