FastQCFastQC Report
Tue 5 Jul 2016
500523_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500523_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207110
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTT1002.07001E-9122.643616235
TATACCC303.322541E-4112.939155
TGACAGA850.001969507272.1433235
TATTGAA600.00520454256.4695742
TAATGGT600.00520454256.4695744
CTATGAA650.007143270752.125762
GTACCGT1803.2428183E-431.3719866
CCTATGC1500.00504068930.1171048
TCAAGAT1650.00802912827.3791873
CAGATAG2800.0042603320.1677065
GCAGAGC3200.00921070317.6467423
GGGCCAC6350.002451569512.4499846
GGGGGGG18908.781637E-49.733621235
AGCACCG12404.1634738E-49.1079965
GCACCGA12050.00325729028.4352886
ACCGCGC14400.0018367427.84299668
CACCGCG24150.007043575.6118837