FastQCFastQC Report
Tue 5 Jul 2016
500519_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500519_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202470
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG450.001711416674.764252
GCATTAT450.001711416674.764251
GTCATAA550.003791980461.1707461
ATTAATC550.003791980461.1707465
ATCTAAA150.007692860451.171562220-224
TCTGGGG2050.001911814936.693962235
AAGGGGG3003.2525964E-431.342758235
TATTCCG1700.00961145226.3873835
CAGCCTG5353.7347432E-516.769555
GTCCTGG4400.003288348715.2926861
GGTGGGG11551.9433523E-514.653756235
GATACCG4950.007187044213.5934995
TCCAGCA6400.002740828312.2660092
CCGCTTT1450.004353159611.537627160-164
CCGGGGG13900.001513002210.823399235
CGGGGGG20804.4472204E-49.04118235
GGGGGGG50901.62168E-66.6503296235