FastQCFastQC Report
Tue 5 Jul 2016
500519_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500519_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202470
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAATC150.006517168149.120732
CTGTCTT900.002728313266.45097235
TTTATGG550.00383321861.0039252
CAAATGA1050.005024304656.957977235
GTGTAGA1150.0072006852.005116235
GTAAAAG650.007424516651.6187133
GTGTATC1059.187932E-442.605921
CCTCTAC1300.00262378534.4124761
ATACGCA1350.003156958833.137944
GTCTATT300.0047572430.486995-99
CTCCAAC1955.487594E-428.6770632
ACAGTCC1600.00724678627.9601368
TCCAACC2159.709123E-426.0094283
GTCCAAG2750.00406146520.3346421
TTGTTGT3557.961447E-418.9026264
GTAGACG3150.00889854417.7524684
ATGACCA4050.001926193516.568975
GTGATGG6251.4418818E-414.315591
TGTGGTG5100.00891448913.157715