FastQCFastQC Report
Tue 5 Jul 2016
500024_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500024_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199583
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATAA300.00933232132.21576235
CAGTCAT450.002001167571.873694
CACATAA850.002219890269.99658235
GTATAGA550.00443271458.8057441
TAAACAA1200.0086950349.580914235
CTTATAA1700.003762600723.332195235
TACAAAT856.4118905E-423.332195235
GCCCATG4552.795349E-416.5862337
GGGGTGG10700.001788400912.974443235
AGGGGGG5151.7812163E-611.552834235
TCTTATA3601.2281998E-711.11092230-234
GTGCTGC7900.001414555410.9175211
GGGGGGG37903.8198777E-1110.465628235
GTCTCTT3450.00132247128.114885225-229
CTCTTAT3300.0066929367.7920737230-234
GGGGGGT7101.6046624E-46.0361133230-234