FastQCFastQC Report
Tue 5 Jul 2016
500024_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500024_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199583
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTT1103.634714E-581.85196235
TCTTTTT950.002087650871.08196235
GCACATG1451.4280446E-462.094585235
TTTTCAA1250.006191418554.02229235
GTGTAGG1300.00327329532.893851
ACACTGA1300.00327329532.893851
CCTTACC1350.003937781331.675569
TACTGGT1350.003937781331.675562
GTACTGG2756.1597366E-627.2121851
GCCTACA1600.00903145626.7262522
CTGCTCA3357.3015783E-419.1471654
GTGGTTC2900.00716587718.43199
CACGCGG4900.00340122269.187465235
CAGCACC13200.00133774248.0988654
AAAAAAA4100.005317497.0404563220-224