FastQCFastQC Report
Tue 5 Jul 2016
500005_S17_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500005_S17_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233599
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATAA900.00323789563.64753235
TAATATC600.00569648255.197542
CCCATAA1150.001532622438.398291
TAGAATC300.005043613330.1250575-79
AGATTTA350.005119011330.033352225-229
GTAGGGG2800.00816628327.277512235
ATTGAAC1650.00897513826.7624423
TAGCTCG1650.00897513826.7624425
TTATACC550.00159779823.890165225-229
CCGTTCA2850.00538649219.3675589
GGTCAAA3100.00875041617.8056581
CTTATAA1150.006098199616.603704235
TACACCG4000.00193495516.5592615
GGATCAC5004.7901817E-415.455311
TTATACA2500.001155948515.275407235
AAGACAG1350.001100131313.856502230-234
TCAACAC6502.2485429E-413.5870873
GGACCGA5100.0097197312.9876566
GTCCCGG6903.726207E-412.799431
TGGACCG6150.002289163612.5652925