FastQCFastQC Report
Tue 5 Jul 2016
500005_S17_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500005_S17_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233599
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG150.0068783322146.459531
TATATAG150.0068783322146.459532
CTATATA251.7963763E-4131.813581
CATTAAT303.7114488E-4109.844652
GCATTAA356.8509695E-494.152551
ACCATAA450.001858546773.229771
CACCATA950.002685689466.71827235
AGTAATG500.002822445765.906795
TTAGTTT400.007687607351.174633230-234
GTATAGG650.00797382850.6975331
ATGTAGG1200.001935871436.6148831
TGTACTG1300.002866848433.7983555
GTGTATG1450.004893345330.3019731
TTTTTTT902.939114E-622.530752195-199
GCCCCAT2800.00500513219.6151161
GATGTAC2800.00500513219.6151163
GTGTTGC4853.9469174E-415.8538671
GGTGGGG8250.003176251415.365418235
GGAGGTG4650.00545259814.1735035
GCAGTGG4750.006278483713.8751138