FastQCFastQC Report
Thu 30 Jun 2016
Undetermined_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324136
Sequences flagged as poor quality0
Sequence length50-241
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCGT550.00340760162.8522346
TAATGGG1600.00919012948.89894235
CCGTCTA1300.00226845635.455119
AGGCTGA1300.00226845635.455116
CTAGTAC1450.003873998531.7873383
GTATAGG1450.003873998531.7873381
TTATAGT2652.5818736E-630.4378744
CCATGGA1650.00728589127.9343242
CTATATA2107.1179826E-427.43551
CCGATCA3006.780456E-626.886799
CGTATAC1750.00970791326.3380783
TATACAT2901.663665E-423.8405045
TATAGTA2500.00196705823.045825
CTAATAC3552.4990264E-522.721233
TAATACT3602.784177E-522.4056574
TAGATAC3556.524537E-419.475343
ATATAGA3607.1685086E-419.2048472
ATAATAC3300.00980749217.4589543
TTAGATG4902.968434E-416.46134
TTATACA4300.002358532516.0784784