FastQCFastQC Report
Thu 30 Jun 2016
MU585039B_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU585039B_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518625
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAC1200.001616176137.994363
GTCTAAG2606.128539E-835.0717161
TAGGCCC1400.003442649332.5665974
GGCGTGT1450.004087996731.4436078
GCGTGTT2056.593814E-427.800759
ACTCCAC2506.54099E-527.355948
GACCCCT1700.00889216426.8195486
GCTCGGG1158.591973E-426.572279210-214
GTATAGA5809.094947E-1225.547931
ATAACAC5501.2914825E-1024.8690363
CTATACT5553.8544385E-922.5912424
TATAGTG4653.9649422E-722.0612415
GCTAAGG3253.875684E-421.043032
TGGACCG2750.003646270420.7241955
ACATAGA4456.677801E-620.491341
GTATAGG3405.254607E-420.114661
ACAGACC3455.797795E-419.8231458
CCTGTAC4659.764339E-619.6099933
GTATACA4701.0708793E-519.4013751
ATTATAC10600.019.3556163