FastQCFastQC Report
Thu 30 Jun 2016
MU585039B_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU585039B_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518625
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC1600.00655915928.5452188
CCCTAGA2056.5273145E-427.8489912
TACACCG5850.027.3253365
GTATACA4654.8567017E-1027.0105291
TCTACAC5358.913048E-1125.6106623
ACCGCTC2250.001124117625.3735288
GTCTAGG2400.001637249123.7876821
CCGCTCA2500.002076068222.8361769
CCTATAG2550.00232927122.3884051
TATGACC3703.696765E-521.6017864
GATTAGG2750.003609822820.7601592
TAGACTG2800.004006967420.3894425
ACTGTAC4507.2458333E-620.2988228
GAGTCAG3405.193557E-420.1495657
CCCTGTA2850.004439304620.0317332
GTATATG6952.3392204E-919.7146821
GTCTAGT4108.147374E-519.4942951
TTAGGCT3556.94614E-419.2981764
CCTACAC3607.63145E-419.0301463
GAGCAGG4209.803394E-519.0301466