FastQCFastQC Report
Thu 30 Jun 2016
MG394676V_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394676V_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501099
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG700.00919651448.908275
GTATAGG2909.276846E-1139.3514821
TACCGTG2309.225314E-734.731967
TCTAGGG1550.005631403529.4501423
GTAGACT2905.6248846E-627.546044
GTACACA3201.2078686E-524.9635961
ACCGTGT2300.001281755224.8085448
CTGCCGT2550.002336471622.3763359
GTATGGG3754.116643E-521.302271
TACAGTG4304.911215E-621.2314997
GTATGTA3253.844304E-421.0681781
GACACCG2750.003620954520.7489625
GTGTAGG2750.003620954520.7489621
TATACAG7002.57387E-919.5633095
ACCTGTG2950.00543586119.3422552
TCTACAC4751.1611088E-519.2200933
ACTCAGG3000.00599020219.0198846
GTACCGT3658.402928E-418.7593366
CTTATAC6152.7773058E-718.5559833
GTATACA4951.656762E-518.4435231